Related Experiment Video
Updated: Jul 7, 2026

Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes
Published on: February 24, 2017
Morphological observation of nuclear sub-localization of human angiogenin in HUVECs
Jie Wu1, Hong-Bin Zhang, Jie Wang
1Department of Medical Research, Guangzhou Liuhuaqiao Hospital, Liuhua road 111, Guangzhou, Guangdong Province, 510010, P.R. China.
Abstract:
Angiogenin, a potent angiogenic factor, was cloned and expressed by Escherichia coli and then purified with gel filtration chromatography. Approximately 90% pure angiogenin was obtained to generate a monoclonal antibody. Using western immunoblotting and ELISA, we confirmed that monoclonal antibody C46 secreted from hybridoma cells stably and specifically binds to angiogenin. The fused protein angiogenin-EGF was then expressed in HUVECs, and the subcellular localization of the recombinant protein was determined by confocal microscopy and TEM assay. Recombinant angiogenin was found to mainly concentrate in the pars granulosa of the nucleus, where the protein accumulates to form ribonucleoprotein particles.
More Related Videos
Related Concept Videos
Mechanism of Angiogenesis
Nuclear Localization Signals and Import
Regulation of Angiogenesis and Blood Supply

