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Changes in cyclic adenosine monophosphate-responsive element binding proteins in rat hepatomas.

J Kwast-Welfeld¹, I de Belle, P R Walker

¹Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada.

Cancer Research

|January 15, 1991

Summary

A specific cyclic adenosine monophosphate (cAMP)-responsive element (CRE) binding (CREB) protein, crucial for gene expression, was lost in Morris hepatomas. This loss is linked to neoplastic properties, not cell growth, potentially causing abnormal gene activity.

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Area of Science:

Molecular Biology
Cancer Research
Biochemistry

Background:

Cyclic adenosine monophosphate (cAMP)-responsive element (CRE) binding (CREB) proteins regulate gene expression.
Morris hepatomas are chemically induced rat liver tumors used in cancer research.

Purpose of the Study:

To investigate changes in CREB proteins in Morris hepatomas.
To identify the specific CREB protein lost in these tumors and its potential role.

Main Methods:

Southwestern blotting
Western blotting
Gel retardation assays
Nuclear protein extraction
Phosphorylation and dephosphorylation experiments

Main Results:

Related Experiment Videos

Six CRE-binding proteins were identified in normal rat liver cells, with a Mr 47,000 protein showing high CRE specificity.
The Mr 47,000 CREB protein was absent in 5123tc and 5123D Morris hepatomas.
Loss of the Mr 47,000 CREB protein was independent of cell proliferation, as observed during liver regeneration.

Conclusions:

The Mr 47,000 protein is a distinct CREB family member crucial for cAMP-mediated gene expression.
Its absence in Morris hepatomas suggests a link to neoplastic properties and may cause aberrant gene expression.