[Detection of Y chromosome microdeletions in AZF region by liquid chip technology]
Si-yao Liu1, Xian-ping Ding, Xia Wei
1Sichuan University, Sichuan, 610064 People's Republic of China.
Objective:
To establish a liquid chip technology to detect Y chromosome microdeletions in Chinese infertile males with azoospermia or oligozoospermia.
Methods:
Multiplex PCR and liquid chip technology were used to detect the Y chromosome microdeletions in AZF region in 178 infertile patients with azoospermia and 134 infertile patients with oligozoospermia as well as 40 fertile control men.
Results:
Forty out of 312 patients (12.8%) were found to have deletions in AZF region. The microdeletion frequency was 14%(25/178) in the azoospermic group, 9.6%(11/114) in the oligospermic and 20%(4/20) in the severe oligospermic group.
Conclusion:
The authors developed a high-throughput, fast and simple assay to screen the AZF region microdeletions of Y chromosome.
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