Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 7, 2026

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
06:58

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

Sensitive molecular diagnostics using volume-amplified magnetic nanobeads.

Mattias Strömberg1, Jenny Göransson, Klas Gunnarsson

  • 1Department of Engineering Sciences, Division of Nanotechnology and Functional Materials, Uppsala University, The Angström Laboratory, Box 534, SE-751 21 Uppsala, Sweden.

Nano Letters
|February 6, 2008
PubMed
Summary

This study introduces a novel DNA detection method using magnetic nanobeads. This technique enables highly sensitive, cost-efficient diagnostics by measuring changes in magnetic properties after DNA amplification.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fast hydrated-ion transport and desolvation in pyridinyl COF membranes <i>via</i> competitive coordination.

Chemical science·2026
Same author

Tailoring Biomass-Derived Organosolv Lignin Derivatives for High-Capacity Adsorption of Rhodamine B.

ChemSusChem·2026
Same author

Retraction notice to "Corrigendum to "Impact of polymer chemistry on critical quality attributes of selective laser sintering 3D printed solid oral dosage forms" [International Journal of Pharmaceutics:X Volume 6 (2023) 100203] [International Journal of Pharmaceutics: X 9 (2025) 100319].

International journal of pharmaceutics: X·2026
Same author

Geometry impact on fundamental properties of theophylline-containing SLS printed pharmaceutical tablets.

Frontiers in drug delivery·2025
Same author

Scalable and Versatile Fabrication of Free-Standing Covalent Organic Framework Membranes with Tunable Microstructure for Molecular Separation.

Journal of the American Chemical Society·2025
Same author

Spatially resolved microRNA expression in tissues: technologies, challenges, and opportunities.

Trends in genetics : TIG·2025

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • Current diagnostic tools often rely on fluorescence, which can be expensive and complex.
  • There is a need for cost-effective, high-sensitivity methods for DNA sequence detection.

Purpose of the Study:

  • To develop a novel, non-fluorescence-based diagnostic principle for DNA sequence detection.
  • To demonstrate the use of magnetic nanobeads for sensitive DNA quantification.

Main Methods:

  • Utilizing single-stranded oligonucleotide-tagged magnetic nanobeads for DNA detection.
  • Employing circularization of linear padlock probes followed by rolling circle amplification (RCA) for target DNA volume amplification.
  • Analyzing changes in the magnetization spectrum of nanobeads upon hybridization with amplified DNA coils.

More Related Videos

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads
07:13

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example
08:10

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example

Published on: May 29, 2012

Related Experiment Videos

Last Updated: Jul 7, 2026

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
06:58

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads
07:13

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example
08:10

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example

Published on: May 29, 2012

Main Results:

  • Demonstrated a dramatic change in the magnetization spectrum of nanobeads when hybridized with RCA coils.
  • Achieved concentration determination of RCA coils down to the picomolar (pM) range.
  • Showed that bead incorporation into coils is diffusion-controlled and can be accelerated by higher temperatures.

Conclusions:

  • The developed method offers a new principle for non-fluorescence-based, cost-efficient, high-sensitivity diagnostics.
  • Magnetic nanobeads coupled with RCA provide a powerful tool for sensitive DNA detection and quantification.
  • Optimizing incubation conditions can enhance the speed and efficiency of this diagnostic approach.