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Related Experiment Video

Updated: Jul 7, 2026

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development
06:55

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

Published on: January 26, 2017

Long-term time-lapse fluorescence imaging of developing zebrafish.

Makoto Kamei1, Brant M Weinstein

  • 1Laboratory of Molecular Genetics, National Institute of Child Health and Human Development (NICHD), National Institutes of Health, Bethesda, Maryland 20892, USA.

Zebrafish
|February 6, 2008
PubMed
Summary

Researchers developed a novel imaging chamber for long-term, dynamic visualization of transgenic zebrafish development. This new tool enables extended time-lapse imaging, overcoming limitations of previous methods for studying organ and tissue development in living animals.

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Area of Science:

  • Developmental Biology
  • Zebrafish Research
  • Microscopy Techniques

Background:

  • Transgenic zebrafish expressing fluorescent proteins allow dynamic visualization of organ and tissue development.
  • Existing time-lapse imaging methods are limited for long-term studies due to poor environmental control (temperature, oxygen, evaporation).

Purpose of the Study:

  • To develop and validate a new imaging chamber for long-term, high-resolution time-lapse imaging of transgenic zebrafish.
  • To overcome the limitations of current methods for maintaining zebrafish health and developmental integrity during extended imaging periods.

Main Methods:

  • A novel imaging chamber was designed to provide continuously circulating, warm, and oxygenated aqueous media.
  • Multiphoton time-lapse imaging was performed on Fli1-EGFP transgenic zebrafish to visualize developing trunk blood vessels.

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Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
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Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos
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Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

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Last Updated: Jul 7, 2026

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development
06:55

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

Published on: January 26, 2017

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
07:28

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

Published on: February 27, 2014

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos
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Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

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  • The chamber's performance was assessed over 5 days for developmental effects, viability, and circulatory flow strength.

    • Main Results:

    • The new imaging chamber successfully supported 5-day multiphoton time-lapse imaging of developing zebrafish blood vessels.
    • No significant developmental delay, loss of viability, or reduction in circulatory flow strength was observed in the fish during imaging.
    • The chamber effectively controlled environmental conditions, preventing anoxia and evaporation.

    Conclusions:

    • The developed imaging chamber is a valuable new tool for long-term dynamic imaging of transgenic zebrafish.
    • This technology facilitates advanced studies of developmental processes in vivo over extended periods.
    • It enables robust, non-invasive observation of organogenesis and tissue development in zebrafish models.