Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

Inhibitors of Viral Protein Synthesis

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...

Microorganisms in Medicine and Therapeutics

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

INTRA-OPERATIVE ASSESSMENT OF TIBIAL PLATEAU FRACTURE REDUCTION IN LOW-RESOURCE SETTINGS.

Georgian medical news·2025

Same author

Seroprevalence and molecular characterization of lumpy skin disease virus in Bahawalpur district of South Punjab, Pakistan.

Polish journal of veterinary sciences·2025

Same author

Molecular detection and therapeutic study of Trypanosoma brucei evansi from naturally infected horses in Punjab, Pakistan.

Polish journal of veterinary sciences·2022

Same author

Sporadic malignant peripheral nerve sheath tumour (MPNST) in a 3-year-old girl: A diagnostic challenge.

The Malaysian journal of pathology·2022

Same author

<i>In vitro</i> and <i>in vivo</i> evaluation of some antimicrobials and disinfectants against bacterial pathogens from hoof lesions in dairy cattle.

Iranian journal of veterinary research·2021

Same author

Petroleum pipeline monitoring using an internet of things (IoT) platform.

SN applied sciences·2021

Search research articles

Related Experiment Video

Updated: Jul 7, 2026

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Published on: September 5, 2016

Review article: RNA interference--potential therapeutic applications for the gastroenterologist.

R S Pellish¹, A Nasir, B Ramratnam

¹Division of Gastroenterology, Warren Alpert Medical School of Brown University, Rhode Island Hospital, Providence, RI 02903, USA.

Alimentary Pharmacology & Therapeutics

|February 6, 2008

Summary

RNA interference (RNAi) offers a new way to control gene expression for treating gastrointestinal and liver diseases. Further research into delivery and side effects is needed for clinical use.

More Related Videos

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions

Published on: April 21, 2016

DNA Vector-based RNA Interference to Study Gene Function in Cancer

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

Related Experiment Videos

Last Updated: Jul 7, 2026

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Published on: September 5, 2016

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions

Published on: April 21, 2016

DNA Vector-based RNA Interference to Study Gene Function in Cancer

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

Area of Science:

Molecular Biology
Genetics
Gastroenterology

Background:

RNA interference (RNAi) is a gene regulation technique using short double-stranded RNA.
RNAi selectively inhibits gene expression post-transcriptionally.
Gastrointestinal and hepatic disorders are promising targets for RNAi therapy due to drug delivery advantages.

Purpose of the Study:

To review literature on RNAi applications in gastroenterology.
To explore future RNAi-based therapies for luminal diseases.

Main Methods:

Literature search using PubMed with the term 'RNA interference'.
Focus on studies related to therapeutic uses in gastrointestinal and liver diseases.

Main Results:

Cellular and animal models show potential for short-interfering RNA (siRNA) in treating viral hepatitis and inflammatory bowel disease.
siRNA-based therapies may offer a new frontier in molecular-targeted treatments for gastroenterology and hepatology.

Conclusions:

Short-interfering RNA (siRNA) enables specific gene expression inhibition.
Clinical translation requires further investigation of side effects and delivery systems.
Mucosal or endoscopic delivery of siRNA may lead to early clinical applications.