Related Experiment Video
Updated: Jul 7, 2026
Production and Testing of Antimicrobial Peptides and Their Mimics
Published on: April 10, 2026
Eukaryotic expression and antimicrobial spectrum determination of the peptide tachyplesin II
Feng Xu1, Kun Meng, Ya-Ru Wang
1Microbial Engineering Department, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12, Zhongguancun South Street, Haidan District, Beijing 100081, China; College of Life Science, Yangtze University, Jingsha, Hubei 434100, China.
Abstract:
Ta0-a, the gene encoding the mature antimicrobial peptide tachyplesin II, was engineered to optimize the coding sequence according to codon usage bias in yeast. Ta0-a was efficiently expressed in the methylotrophic yeast Pichia pastoris strain SMD1168. The recombinant peptide Ta0 reached 150mg/L after methanol induction for 6 d. Ta0 was rapidly purified to homogeneity by a single step of size-exclusion chromatography. The minimal lethal concentrations of Ta0 to the Escherichia coli strain K12 was 30 microg/mL. Ta0 exhibited a wide range of antimicrobial activity: the growth of 26 bacterial and fungal strains, including some typical food/feed spoilage microorganisms, was all substantially inhibited. This result indicates the potential practical application of the recombinant peptide in various industrial products.
