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Updated: Jul 7, 2026
Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
Published on: February 1, 2017
[Construction and antigenic evaluation of a recombinant MVA virus-like particle expressing HBV C gene]
Xiang-ling Luan1, Wei Kong, Su-jun Liu
1Postgraduate Medical School of PLA, Beijing 100853, China. luanxiangling@sohu.com
Objective:
To construct the virus-like parcel expressing hepatitis B virus (HBV) C gene and identify its immunogenicity.
Methods:
HBV C gene was cloned into the shuttle vector pSC11, and the resulted plasmid pSC11-C was transfected into modified vaccinia virus Ankara (MVA).
Results:
pSC11-C was correctly constructed as verified by sequence analysis and PCR, and the recombinant virus-like parcel possessed good immunogenicity.
Conclusion:
The MVA-C expressing HBV C gene has been successfully constructed to provide important basis for gene therapy research of chronic HBV infection.
