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Updated: Jul 7, 2026
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
The comparison of thiobarbituric acid reactive substances (TBARS) concentrations in plasma and serum from dairy
Mari Aoki1, Tomoko Oshita, Minoru Sakaguchi
1National Agricultural Research Center for Hokkaido Region, Hokkaido, Japan. mari@affrc.go.jp
Abstract:
The aim of this study was to compare thiobarbituric acid reactive substances (TBARS) concentrations in serum, plasma with heparin (heparin plasma), and plasma with ethylenediaminetetraacetic acid disodium salt (EDTA plasma) as anticoagulants from dairy cattle. Serum, heparin plasma, and EDTA plasma TBARS were not sufficiently strongly correlated to allow accurate prediction of one set of values from the other. Heparin plasma TBARS concentrations were found to be lower, and were affected by the duration of mixing during the assay process. The results suggest that it is necessary to differentiate TBARS concentrations between different sample types such as serum, heparin plasma, and EDTA plasma. For measurements of TBARS concentrations in cattle, EDTA plasma samples may be more suitable than the other samples.
