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Updated: Jul 7, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
Published on: February 8, 2014
Image reconstruction by backprojection from frequency-domain optical measurements in highly scattering media
Abstract:
The reconstruction of the location and optical properties of objects in turbid media requires the solution of the inverse problem. Iterative solutions to this problem can require large amounts of computing time and may not converge to a unique solution. Instead, we propose a fast, simple method for approximately solving this problem in which calculated effective absorption and reduced scattering coefficients are backprojected to create an image of the objects. We reconstructed images of objects with centimeter dimensions embedded in a diffusive medium with optical characteristics similar to those of human tissue. Data were collected by a frequency-domain spectrometer operating at 120 MHz with a laser diode light source emitting at 793 nm. Intensity and phase of the incident photon density wave were collected from linear scans at different projection angles. Although the positions of the objects are correctly identified by the reconstructed images, the optical parameters of the objects are recovered only qualitatively.
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