Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Portable Acceleration of CMS Computing Workflows with Coprocessors as a Service.

Computing and software for big science·2024
Same author

Measurement of Energy Correlators inside Jets and Determination of the Strong Coupling α_{S}(m_{Z}).

Physical review letters·2024
Same author

Observation of the ϒ(3S) Meson and Suppression of ϒ States in Pb-Pb Collisions at sqrt[s_{NN}]=5.02  TeV.

Physical review letters·2024
Same author

Search for Narrow Trijet Resonances in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2024
Same author

Combination of Measurements of the Top Quark Mass from Data Collected by the ATLAS and CMS Experiments at sqrt[s]=7 and 8 TeV.

Physical review letters·2024
Same author

Search for Baryon Number Violation in Top Quark Production and Decay Using Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2024
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jul 7, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
09:16

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Commercial spectrophotometer for particle sizing.

F Ferri1, A Bassini, E Paganini

  • 1Institute of Mathematical, Physical and Chemical Sciences, University of Milan at Como, via Lucini, 3-22100 Como, Italy.

Applied Optics
|February 1, 1997
PubMed
Summary
This summary is machine-generated.

This study accurately determined polystyrene particle size and concentration in water using spectral extinction data. A modified spectrophotometer and nonlinear algorithm enabled precise measurements for particle sizes between 0.6-2.8 micrometers.

More Related Videos

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media
05:16

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

Published on: October 25, 2021

A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer (SMPS-ICPMS)
11:18

A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer (SMPS-ICPMS)

Published on: July 11, 2017

Related Experiment Videos

Last Updated: Jul 7, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
09:16

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media
05:16

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

Published on: October 25, 2021

A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer (SMPS-ICPMS)
11:18

A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer (SMPS-ICPMS)

Published on: July 11, 2017

Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Optical Physics

Background:

  • Accurate characterization of particle size distribution and concentration is crucial in various scientific and industrial applications.
  • Traditional methods for particle analysis can be limited by accuracy, precision, or sample preparation requirements.
  • Spectral extinction measurements offer a non-invasive approach to probe particle properties.

Purpose of the Study:

  • To develop and validate a method for accurately recovering particle-size distribution and concentration of polystyrene particles in water.
  • To assess the performance of a modified commercial spectrophotometer coupled with a nonlinear iterative algorithm for this purpose.
  • To establish the reliable range of particle sizes and concentrations that can be analyzed.

Main Methods:

  • Utilized spectral extinction data measured by a commercial spectrophotometer modified with a spatial filter.
  • Employed a nonlinear iterative algorithm for inverting the spectral extinction data.
  • Investigated the wavelength range of 0.3-1.1 micrometers for extinction coefficient measurements.

Main Results:

  • Successfully recovered particle-size distribution and concentration of polystyrene particles with high accuracy.
  • Achieved precise recovery of average diameters (better than +/-1%) and accurate concentration measurements (approx. 5%).
  • Demonstrated reliable retrieval of particle distributions in the diameter range of 0.6-2.8 micrometers across various concentrations.

Conclusions:

  • The developed method enables accurate and precise characterization of particle size and concentration using spectral extinction.
  • Modification of a commercial spectrophotometer and application of a nonlinear iterative algorithm significantly enhance measurement capabilities.
  • This technique provides a robust tool for analyzing microparticle suspensions within specific size and concentration ranges.