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Enteric bacteria and ingested inert particles translocate to intraperitoneal prosthetic materials.
E M Mora1, M A Cardona, R L Simmons
1Department of Surgery, University of Pittsburgh, PA 15261.
Archives of Surgery (Chicago, Ill. : 1960)
|February 1, 1991
Summary
Sterile biomaterials implanted in the abdomen attracted enteric bacteria, leading to contamination. This suggests inflammation can cause bacteria to move from the gut to implants through the intact bowel wall.
Area of Science:
- Biomaterials Science
- Microbiology
- Surgical Research
Background:
- Prosthetic devices often utilize sterile biomaterials.
- The risk of bacterial contamination of implanted biomaterials is a significant concern.
Purpose of the Study:
- To investigate bacterial translocation to intraperitoneal biomaterials.
- To determine if sterile reactive stimuli influence bacterial translocation.
Main Methods:
- Implantation of biomaterials (Dacron velour, woven Dacron, Biomer, cotton) into the mouse peritoneum.
- Monitoring for bacterial contamination of biomaterials and mesenteric lymph nodes.
- Tracking ingested fluorescent beads to assess particle translocation.
Main Results:
- Intraperitoneal biomaterials became contaminated with enteric bacteria within 24-72 hours.
- Bacterial translocation to mesenteric lymph nodes paralleled biomaterial contamination.
- Subcutaneous implants remained sterile; peritoneal contamination depended on biomaterial contamination.
- Ingested particles translocated to enterocytes, macrophages, and intraperitoneal biomaterials.
Conclusions:
- Intraperitoneal sterile stimuli can induce bacterial translocation to prostheses via the intact bowel wall.
- Phagocytosis within the bowel wall and chemotaxis to inflammatory sites appear to be mechanisms involved.