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Related Experiment Videos

Abscisic acid induces a cytosolic calcium decrease in barley aleurone protoplasts.

M Wang1, B Van Duijn, A W Schram

  • 1Centre for Phytotechnology RUL/TNO, Department of Molecular Plant Biotechnology, Leiden, The Netherlands.

FEBS Letters
|January 14, 1991
PubMed
Summary

Abscisic acid (ABA) triggers a rapid decrease in plant cell calcium levels. This ABA-induced calcium reduction is mediated by the plasma membrane calcium pump, not calcium channels.

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Area of Science:

  • Plant Physiology
  • Cell Signaling
  • Biochemistry

Background:

  • Plant hormones like abscisic acid (ABA) regulate crucial physiological processes.
  • Cytosolic calcium ions (Cai) act as vital second messengers in plant signal transduction pathways.
  • Understanding calcium dynamics is key to deciphering plant hormone action.

Purpose of the Study:

  • To investigate the effect of abscisic acid (ABA) on cytosolic calcium concentrations (Cai) in barley aleurone protoplasts.
  • To elucidate the mechanism underlying ABA-induced changes in Cai.
  • To explore the role of ABA-mediated calcium signaling in plant responses.

Main Methods:

  • Measurement of Cai using the calcium-sensitive fluorescent dye Indo-1 in barley aleurone protoplasts.
  • Application of varying concentrations of ABA to stimulate protoplasts.

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  • Treatment with calcium (Ca2+) antagonists (La3+, Cd2+) and channel blockers (verapamil, nifedipine) to assess their effects.

    • Main Results:

    • Basal Cai levels were approximately 200 nM.
    • ABA stimulation caused a rapid, dose-dependent decrease in Cai to around 50 nM within 5 seconds.
    • Lanthanum (La3+) and cadmium (Cd2+) inhibited the ABA-induced Cai decrease, while verapamil and nifedipine did not.

    Conclusions:

    • ABA induces a rapid decrease in cytosolic calcium in barley aleurone protoplasts.
    • The results suggest ABA activates the plasma membrane Ca2(+)-ATPase, leading to calcium efflux.
    • This ABA-mediated calcium decrease may play a role in ABA signal transduction and antagonizing gibberellic acid effects.