Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Poly(DADMAC) incorporated lipid nanoparticles enhance the delivery of antimicrobial peptides into plant cells.

Scientific reports·2026
Same author

Developmental regulation and physical interaction among enzymes involved in sorgoleone biosynthesis.

The Plant journal : for cell and molecular biology·2023
Same author

Reciprocal Regulation of BRN2 and NOTCH1/2 Signaling Synergistically Drives Melanoma Cell Migration and Invasion.

The Journal of investigative dermatology·2021
Same author

Objective determination of peripheral edema in heart failure patients using short-wave infrared molecular chemical imaging.

Journal of biomedical optics·2021
Same author

Correction: Challenges associated with homologous directed repair using CRISPR-Cas9 and TALEN to edit the DMD genetic mutation in canine Duchenne muscular dystrophy.

PloS one·2020
Same author

Creation and characterization of an immortalized canine myoblast cell line: Myok9.

Mammalian genome : official journal of the International Mammalian Genome Society·2020

Related Experiment Video

Updated: Jul 7, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Protein screening using cold microwave technology.

Aaron G Smith1, Carol B Johnson, E Ann Ellis

  • 1Microscopy and Imaging Center, Texas A&M University, College Station, TX 77843, USA.

Analytical Biochemistry
|February 7, 2008
PubMed
Summary

This study introduces a novel cold microwave technology protocol that significantly reduces protein detection time to under one hour for dot and Western blots. This method offers lower background noise and maintains signal strength, improving laboratory efficiency.

More Related Videos

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
07:22

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

Related Experiment Videos

Last Updated: Jul 7, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
07:22

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Laboratory Science

Background:

  • Protein detection methods like dot and Western blots are crucial but time-consuming in research.
  • Conventional protocols often require lengthy incubation and processing times, impacting laboratory throughput.

Purpose of the Study:

  • To develop and validate a rapid protocol for protein detection using cold microwave technology.
  • To assess the efficiency, signal strength, and background noise of the new method compared to traditional approaches.

Main Methods:

  • Application of a novel cold microwave technology protocol for dot and Western blot assays.
  • Comparison of processing times, reagent usage, and signal-to-noise ratios with conventional methods.
  • Investigation of microwave radiation effects at constant temperatures (21°C) and combined thermal effects at elevated temperatures (37°C).

Main Results:

  • Total processing time for dot and Western blots was reduced to less than one hour.
  • The microwave-assisted protocol yielded lower background noise while maintaining comparable signal strength.
  • Reagent use was decreased in dot blotting, and prestained marker visibility was preserved in Western blots.

Conclusions:

  • Cold microwave technology offers a significantly faster and efficient alternative for protein detection assays.
  • This method is particularly advantageous for large-scale screening of expressed proteins using microwave-assisted dot blotting.
  • The protocol demonstrates potential for enhancing laboratory productivity without compromising data quality.