Related Experiment Video

Updated: Jul 7, 2026

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media
05:16

UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media

Published on: October 25, 2021

Plano-concave microcuvette for measuring the absorption coefficient of highly absorbing liquids

M Csete, Z Bor

    Applied Optics
    |April 1, 1997
    PubMed

    More Related Videos

    Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared07:38

    Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

    Published on: January 10, 2025

    Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer10:29

    Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer

    Published on: November 4, 2009

    Related Concept Videos

    UV–Vis Spectrometers01:14

    UV–Vis Spectrometers

    The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
    UV–Vis Spectroscopy: Beer–Lambert Law01:09

    UV–Vis Spectroscopy: Beer–Lambert Law

    The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...
    UV–Vis Spectrum01:30

    UV–Vis Spectrum

    When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs light between the wavelengths of 180–400 nm range, the UV spectrum is obtained, and if it absorbs light in the 400–780 nm wavelength range, the visible spectrum is obtained.     
    The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar absorptivity (ε) or log ε on the y-axis (ordinate)...
    Methods for Studying Drug Absorption: In vitro01:16

    Methods for Studying Drug Absorption: In vitro

    In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
    The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
    Atomic Absorption Spectroscopy: Lab01:21

    Atomic Absorption Spectroscopy: Lab

    For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
     Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...