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Comparative bacterial proteomics: analysis of the core genome concept
Stephen J Callister1, Lee Ann McCue, Joshua E Turse
1Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA.
Plos One
|February 7, 2008
Summary
This study experimentally verified expressed proteins in 17 bacteria, revealing lifestyle differences linked to conserved genes. Proteomics confirmed expressed ancestral traits, offering deeper insights beyond genomic predictions.
Area of Science:
- Microbiology
- Genomics
- Proteomics
Background:
- Comparative bacterial genomics commonly predicts core genomes indicative of common ancestry.
- Experimental validation of predicted core genomes is often not undertaken due to extensive measurement requirements.
Purpose of the Study:
- To experimentally verify the expression of proteins predicted from genomic ortholog comparisons across diverse bacteria.
- To investigate expressed protein content relationships among phenotypically related bacteria to understand lifestyle associations.
Main Methods:
- Utilized an extensive proteome database developed over six years.
- Performed experimental verification of protein expression based on genomic ortholog comparisons.
- Analyzed expressed protein content in 17 environmental and pathogenic bacterial species.
Main Results:
- Successfully verified the expression of proteins predicted from genomic comparisons in 17 bacterial species.
- Observed more exclusive relationships in expressed protein content among phenotypically related bacteria.
- Demonstrated that expressed protein content reflects specific bacterial lifestyles.
Conclusions:
- Proteomics provides experimental validation for genomic predictions of core ancestral genes.
- This study establishes expressed lifestyle differences among conserved genes, moving beyond simple orthologous relationships.
- Proposes a set of expressed ancestral traits supported by experimental proteomic data.
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