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Updated: Jul 7, 2026
Development and Standardization of an Ex Vivo Micromethod for Intracellular Quantification of Vincristine in Primary ALL Cells by LC-MS/MS
Published on: January 23, 2026
A subcelluar proteomic investigation into vincristine-resistant gastric cancer cell line
Yi-Xuan Yang1, Zhu-Chu Chen, Gui-Ying Zhang
1Key Laboratory of Cancer Proteomics of Ministry of Health of China, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Abstract:
Multidrug resistance (MDR) is a major obstacle to successful cancer treatment. To understand the mechanism of MDR better, a subcelluar proteomics approach was used to compare the protein profile between vincristine-resistant human gastric cancer cell line SGC7901/VCR and its parental cell line SGC7901. After differential solubilization, the subfractionation proteins were separate by two-dimensional gel electrophoresis (2-DE), and the differential protein spots were identified by both MALDI-TOF-MS and ESI-Q-TOF-MS. Then the differential expressional levels of partial identified proteins were determined by Western blot analysis. Furthermore, one of the highly expressed proteins in SGC7901/VCR, Sorcin, associated with MDR was analyzed. In this study, the well-resolved, reproducible 2-DE patterns of subfractionation proteins from SGC7901/VCR and SGC7901 were established, and 30 differential proteins between the two cell lines were identified. The functional validation showed that the elevated sorcin expression could contribute considerably to the vincristine resistance in SGC7901/VCR. The 30 differentially expressed proteins could be divided into six groups based on their functions: calcium binding proteins, chaperones, metabolic enzymes, proteins relative to signal transduction, proteins involved in transcription and translation, and transportation proteins, and most of them might be new MDR associated proteins, which have not been detected previously. These data will be valuable for further to study the mechanisms of MDR in human gastric cancer.
Insights
Multidrug resistance (MDR) in gastric cancer is a significant challenge. This study identified 30 differential proteins, including Sorcin, that contribute to vincristine resistance, offering new targets for cancer therapy.
Area of Science:
- Proteomics
- Cancer Biology
- Molecular Oncology
Background:
- Multidrug resistance (MDR) impedes effective cancer treatment.
- Understanding MDR mechanisms in gastric cancer is crucial.
Purpose of the Study:
- To compare protein profiles between vincristine-resistant (SGC7901/VCR) and parental (SGC7901) gastric cancer cells.
- To identify novel proteins associated with MDR in human gastric cancer.
Main Methods:
- Subcellular proteomics approach using two-dimensional gel electrophoresis (2-DE).
- Protein identification via MALDI-TOF-MS and ESI-Q-TOF-MS.
- Differential protein expression analysis using Western blot.
Main Results:
- Established reproducible 2-DE patterns for both cell lines.
- Identified 30 differentially expressed proteins between SGC7901/VCR and SGC7901.
- Elevated Sorcin expression was found to contribute to vincristine resistance.
Conclusions:
- The 30 identified proteins, including Sorcin, may represent novel targets for overcoming MDR in gastric cancer.
- These findings provide valuable insights into the molecular mechanisms of MDR.
- Further research into these proteins could lead to improved cancer treatment strategies.
