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EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
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GeneXpert enterovirus assay: one-year experience in a routine laboratory setting and evaluation on three proficiency

Katja Seme1, Tina Mocilnik, Kristina Fujs Komlos

  • 1Institute of Microbiology and Immunology, Faculty of Medicine, Zaloska 4, Ljubljana 1000, Slovenia. mario.poljak@mf.uni-lj.si.

Journal of Clinical Microbiology
|February 8, 2008
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.