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A role for membrane-bound CD147 in NOD2-mediated recognition of bacterial cytoinvasion

Andreas Till1, Philip Rosenstiel, Karen Bräutigam

  • 1Institute for Clinical Molecular Biology, University Hospital Schleswig-Holstein, Campus Kiel, Schittenhelmstr 12, Kiel, Germany.

Journal of Cell Science
|February 8, 2008
PubMed

Insights

NOD2 receptor mutations link to inflammatory diseases. This study identifies CD147 as a NOD2 partner, influencing its signaling and enhancing bacterial invasion, potentially guiding NOD2 function during infection.

Area of Science:

  • Immunology
  • Cell Biology
  • Microbiology

Background:

  • NOD2 is an intracellular receptor recognizing bacterial muramyl dipeptide.
  • NOD2 mutations are linked to inflammatory diseases like Crohn disease, asthma, and atopic eczema.
  • CD147 (BSG/EMMPRIN) regulates cell migration, differentiation, and inflammation.

Purpose of the Study:

  • To identify protein interaction partners of NOD2.
  • To investigate the functional impact of the CD147-NOD2 interaction on NOD2 signaling.
  • To determine CD147's role in bacterial invasion.

Main Methods:

  • Protein-protein interaction studies to identify CD147 as a NOD2 partner.
  • Analysis of NOD2-dependent signaling pathways.
  • Assays to evaluate the effect of CD147 on Listeria monocytogenes invasion.

Main Results:

  • CD147 was identified as a novel interaction partner of NOD2.
  • The CD147-NOD2 interaction exhibits a complex regulatory effect on NOD2 signaling.
  • CD147 enhances the invasion of Listeria monocytogenes into host cells.

Conclusions:

  • The CD147-NOD2 interaction modulates NOD2's role in innate immunity.
  • CD147 may act as a crucial regulator of NOD2 function at pathogen entry sites.
  • This interaction offers a potential target for managing inflammatory diseases and infections.

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