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M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.

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Related Experiment Video

Updated: Jul 7, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

Linking mitosis with S-phase: Cdc6 at play.

Susanna Boronat1, Judith L Campbell

  • 1Braun Laboratories 147-75, California Institute of Technology, Pasadena, California 91125, USA.

Cell Cycle (Georgetown, Tex.)
|February 8, 2008
PubMed
Summary
This summary is machine-generated.

The Cdc6 protein is crucial for genomic stability, ensuring DNA replication occurs once per cell cycle. It also plays a key role in regulating mitosis by controlling phosphorylation and dephosphorylation.

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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

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Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
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Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

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Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
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Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

Published on: June 14, 2012

Area of Science:

  • Molecular Biology
  • Cell Cycle Regulation
  • Genomic Stability

Background:

  • Maintaining genomic stability requires precise coordination of DNA replication, ensuring each origin fires only once per cell cycle.
  • The pre-replicative complex (pre-RC) is assembled during G1 phase at replication origins, with Cdc6 protein being a key regulated component.
  • Cdc6's role extends beyond DNA replication, impacting events during mitosis.

Purpose of the Study:

  • To review the multifaceted role of the Cdc6 protein in cell cycle regulation.
  • To discuss Cdc6's involvement in phosphorylation and dephosphorylation events critical for mitotic progression.
  • To propose a model linking Cdc6 function to the coordination of DNA replication initiation and mitotic progression.

Main Methods:

  • Review of existing literature on Cdc6 function in DNA replication and mitosis.
  • Analysis of cell cycle-specific phosphorylation and dephosphorylation activities of Cdc6.
  • Development of a theoretical model illustrating Cdc6's role in coupling cell cycle oscillators.

Main Results:

  • Cdc6 is essential for regulated origin firing during DNA replication.
  • Cdc6 participates in regulating phosphorylation and dephosphorylation of substrates vital for mitosis.
  • A model is presented where Cdc6 connects the two major mitotic oscillators.

Conclusions:

  • Cdc6 is a critical regulator linking DNA replication initiation with the precise order of mitotic events.
  • Understanding Cdc6's dual role in replication and mitosis is key to comprehending genomic stability mechanisms.
  • Cdc6 acts as a molecular switch, coupling the cell cycle's replication and division phases.