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Updated: Jul 7, 2026

A Cell-Free Assay Using Xenopus laevis Embryo Extracts to Study Mechanisms of Nuclear Size Regulation
Published on: August 8, 2016
Nuclei size in relation to nuclear status and aneuploidy rate for 13 chromosomes in donated four cells embryos
I E Agerholm1, C Hnida, D G Crüger
1The Fertility Clinic, Braedstrup Hospital, Sygehusvej 20, 8740 Braedstrup, Denmark. inge.e.agerholm@horsens.rm.dk
Purpose:
The aim was to elucidate if the nuclear size and number are indicative of aberrant chromosome content in human blastomeres and embryos.
Methods:
The number of nuclei and the nucleus and blastomere size were measured by a computer controlled system for multilevel analysis. Then the nuclei were enumerated for 13 chromosomes by a combination of PNA and DNA probes.
Results:
In the mononucleated embryos there was no difference in the mean size of chromosomally normal and abnormal nuclei but a significant difference in the mean nuclei size of nuclei that had gained chromosomes compared to nuclei that had lost chromosomes. The nuclei from multinucleated blastomeres had a significant smaller mean size and the frequency of chromosomally aberrant blastomeres was significantly higher.
Conclusion:
The mean nuclear size is not a marker for the chromosome content in mononucleated embryos. However, it seems that the nuclei size can be related to multinucleation and maybe to the chromosome content.
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