Related Experiment Video
Updated: Jul 7, 2026

Co-localization of Cell Lineage Markers and the Tomato Signal
Published on: December 28, 2016
Tissue-specific expression of Cre recombinase from the Tgfb3 locus
Liang-Tung Yang1, Wai-Yee Li, Vesa Kaartinen
1Developmental Biology Program, The Saban Research Institute of Childrens Hospital Los Angeles, Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California 90027, USA.
Abstract:
Tgfb3, a member of the TGF-beta superfamily, is tightly regulated, both spatially and temporally, during embryogenesis. Previous mouse knockout studies have demonstrated that Tgfb3 is absolutely required for normal palatal fusion and pulmonary development. We have generated a novel tool to ablate genes in Tgfb3-expressing cells by targeting the promoterless Cre-pgk-Neo cassette into exon 1 of the mouse Tgfb3 gene, which generates a functionally null Tgfb3 allele. Using the Rosa26 reporter assay, we demonstrate that Cre-induced recombination was already induced at embryonal day 10 (E10) in the ventricular myocardium, limb buds, and otic vesicles. At E14, robust recombination was detected in the prefusion palatal epithelium. Deletion of the TGF-beta type I receptor Alk5 (Tgfbr1) specifically in Tgfb3 expressing cells using the Tgfb3-Cre driver line lead to a cleft palate phenotype similar to that seen in conventional Tgfb3 null mutants. In addition, Alk5/ Tgfb3-Cre mice displayed hydrocephalus, and severe intracranial bleeding due to germinal matrix hemorrhage.
Related Concept Videos
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
TGF - β Signaling Pathway
Reporter Genes
Commonly used reporter...
General Transcription Factors
Transcription Factors

