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Updated: Jul 7, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
[Study of the effect of hepatitis C virus core protein on interferon-induced antiviral genes expression and its
Yan-Zi Chang1, Yan-Chang Lei, You-Hua Hao
1Department of Microbiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Abstract:
To study the effect of HCV core protein on the interferon-induced antiviral genes expression and its mechanisms. Methods HepG2 cells were transiently transfected with HCV core protein expression plasmid and the blank plasmid respectively. RT-PCR was used to analyze the effect of HCV core protein on PKR and 2'-5'OAS expression. The effect of HCV core protein on ISRE-medicated gene expression was detected by luciferase activity assay. Western-blot assay was performed to observe the change of mRNA and protein levels of SOCS3, STAT1 and p-STAT1 following HCV core expression. In the presence of HCV core protein, the transcription of PKR and 2'-5' OAS are down-regulated. ISRE-medicated reporter gene expression and STAT1 phosphorylation were inhibited. The transcription and expression of SOCS3 were induced compared with blank plasmid-transfected group. In HepG2 cells, HCV core protein can down-regulate the expression of some interferon-induced antiviral genes, which involves the induction of SOCS3 and the inhibition of STAT1 phosphorylation.
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