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Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

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Related Experiment Video

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Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

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[Protein array technology applied in high throughput monoclonal antibody generation].

Kai Song¹, Sai Ye, Jia-Jing Zhou

¹National Engineering Center for Biochip at Shanghai, Shanghai Biochip Co., Ltd, Shanghai 201203, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology

|February 9, 2008

Summary

A novel protein array system streamlines monoclonal antibody generation, reducing labor costs and antigen use. This high-throughput method offers an economical and rapid alternative to traditional techniques.

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Last Updated: Jul 7, 2026

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Area of Science:

Biotechnology
Immunology
Protein Array Technology

Context:

Traditional monoclonal antibody generation is labor-intensive and costly.
Screening hybridoma cells is a critical bottleneck in antibody production.
Existing methods require significant amounts of antigen and time.

Purpose:

To develop an efficient, cost-effective, and high-throughput system for monoclonal antibody generation.
To integrate protein array technology with multiplex cloning for improved screening.
To reduce labor costs and antigen consumption in antibody discovery.

Summary:

A new system combining protein array screening with multiplex cloning was established for monoclonal antibody generation.
BALB/c mice were immunized, and positive hybridoma cells were selected using ELISA and then mixed for array screening.
This method yielded 8 monoclonal antibodies in one cycle, using fewer antigens and reducing labor compared to traditional approaches.

Impact:

The developed method serves as an economical, rapid, and simple tool for high-throughput monoclonal antibody generation.
It significantly reduces the time and resources required for antibody discovery.
Facilitates faster development of diagnostic and therapeutic antibodies.