A technique for the quantification of Duddingtonia flagrans chlamydospores in sheep faeces
N F Ojeda-Robertos1, J F J Torres-Acosta, A Ayala-Burgos
1Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Km. 15.5 carretera Mérida-Xmatkuil, Mérida, Yucatán, Mexico.
Abstract:
Previous observations showed that Duddingtonia flagrans chlamydospores were visualized in McMaster chambers containing faeces of treated sheep. This trial explored the McMaster technique as a tool to quantify chlamydospores in sheep faeces. A range of individual chlamydospore doses (from 19.5 x 10(6) to 177.5 x 10(6)) were offered orally to nine lambs for 7 consecutive days. A faecal sample (5 g) was daily obtained from the rectum of each animal (from days 1 to 13) to perform the McMaster technique using a sugar flotation fluid with 1.27 g/mL density. Each chlamydospore counted in the McMaster chamber was considered as 50 chlamydospores per g of faeces (CPG). The results confirmed that the estimated CPG was associated with the daily dose offered to the animals (r(2)=0.90; P<0.001). Furthermore, the total chlamydospore dose received by each animal was strongly associated to the total quantity of CPG obtained from the bulk faeces (TCtot) (r(2)=0.96; P<0.0001). Quantification of CPG can be used as a helpful tool to determine the number of chlamydospores reaching the faeces in orally dosed animals. This could be used to evaluate the efficacy of D. flagrans for the control of gastrointestinal nematode larvae in sheep faeces.
Insights
The McMaster technique accurately quantifies Duddingtonia flagrans chlamydospores in sheep feces. This method helps assess the efficacy of D. flagrans for controlling gastrointestinal nematode larvae in sheep.
Area of Science:
- Veterinary Parasitology
- Mycology
- Animal Science
Background:
- Duddingtonia flagrans chlamydospores have been observed in sheep feces.
- Quantifying chlamydospore presence is crucial for evaluating biological control agents.
- The McMaster technique is a standard method for fecal egg counts in livestock.
Purpose of the Study:
- To validate the McMaster technique for quantifying Duddingtonia flagrans chlamydospores in sheep feces.
- To establish a correlation between administered chlamydospore doses and quantified fecal chlamydospore counts.
- To assess the potential of this quantification method for evaluating D. flagrans efficacy.
Main Methods:
- Oral administration of varying doses of D. flagrans chlamydospores to lambs over 7 days.
- Daily collection of fecal samples (5 g) from each lamb for 13 days.
- Application of the McMaster technique using a sugar flotation fluid (1.27 g/mL density) for chlamydospore enumeration.
Main Results:
- A strong positive correlation was found between the daily chlamydospore dose and the estimated chlamydospores per gram (CPG) in feces (r(2)=0.90; P<0.001).
- The total chlamydospore dose received was highly correlated with the total quantity of CPG obtained from bulk feces (TCtot) (r(2)=0.96; P<0.0001).
- The McMaster technique proved effective in quantifying chlamydospores in sheep feces.
Conclusions:
- The McMaster technique is a reliable tool for quantifying Duddingtonia flagrans chlamydospores in sheep feces.
- Fecal chlamydospore quantification can be used to determine the number of chlamydospores reaching the gastrointestinal tract after oral dosing.
- This method supports the evaluation of D. flagrans efficacy in controlling sheep gastrointestinal nematodes.
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