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Mapping the Cellular Distribution of an Optogenetic Protein Using a Light-Stimulation Grid
Published on: January 26, 2024
White-light schlieren optics using bacteriorhodopsin as an adaptive image grid
Applied Optics
|July 1, 1997
Abstract:
A schlieren apparatus using a bacteriorhodopsin film as an adaptive image grid with white-light illumination is demonstrated for the first time to our knowledge. Relevant spectral properties of the film are characterized. Potential applications include a single-ended schlieren system for gas-leak detection.
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Imaging Biological Samples with Optical Microscopy
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Channel Rhodopsins
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
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