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Related Experiment Video

Updated: Jul 7, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Imaging and tracking of single hyaluronan molecules diffusing in solution.

Tim Kaminski¹, Jan-Peter Siebrasse, Volkmar Gieselmann

¹Institut für Physiologische Chemie, Rheinische Friedrich-Wilhelms-Universität Bonn, Nussallee 11, 53115 Bonn, Germany.

Glycoconjugate Journal

|February 9, 2008

Summary

This summary is machine-generated.

Researchers visualized single hyaluronan molecules in solution, confirming they form random coils. This breakthrough allows detailed study of hyaluronan

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Last Updated: Jul 7, 2026

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Published on: September 5, 2019

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Area of Science:

Biophysics
Biochemistry
Extracellular Matrix Research

Background:

Hyaluronan (HA) is a key extracellular matrix component with diverse biological roles.
Previous studies suggested HA forms random coils in solution, but direct visualization was lacking.

Purpose of the Study:

To visualize and track single hyaluronan molecules in aqueous solution.
To confirm the molecular conformation of hyaluronan in solution.

Main Methods:

Utilized wide-field fluorescence microscopy with laser excitation.
Employed a sensitive EMCCD camera for fluorescence detection.
Tracked single FITC-labeled hyaluronan molecules from rooster comb.

Main Results:

Successfully visualized and tracked individual hyaluronan-FITC molecules in 3D aqueous buffer at room temperature.
Observed Brownian motion consistent with random coil conformation.
Calculated an average diffusion coefficient of D=3.0±0.2 μm²/s.

Conclusions:

Single-molecule tracking confirms hyaluronan forms random coils in solution.
This technique enables high-resolution analysis of hyaluronan organization and cell interactions.
Opens new avenues for studying hyaluronan dynamics and function.