[Effects of Alternaria tenuis nees on Trifolium repens L. under Cu stress]

With pot culture, this paper studied the effects of Alternaria tenuis Nees inoculation on the eco-physiological indices of Trifolium repens L. leaf under Cu stress. The results showed that in the control (not inoculated with the pathogen), the contents of photosynthetic pigments (chlorophyll a, b, a + b and carotenoid) and soluble protein in T. repens leaves decreased markedly with increasing Cu concentration (0-3000 mg x kg(-1)). The enhancement of cellular membrane lipids peroxidation with the increase of Cu concentration led to a rapid accumulation of malondialdehyde (MDA), the damage on cellular membrane structure, and an increase of electric conductivity. The balance of active oxygen metabolism systems was broken, SOD and CAT activities decreased, while POD activity increased. After inoculation with A. tenuis, the damages of Cu on plant membrane systems and active oxygen metabolism systems aggravated, the contents of photosynthetic pigments and soluble protein and the activities of SOD and CAT decreased to different degree, while the electric conductivity, MDA content and POD activity increased markedly, compared with the control.