Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From diabetic foot to dementia: A neurovascular continuum linking systemic diabetic vasculopathy, cerebral small vessel disease, and glymphatic dysfunction.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Bisphenol P triggers hepatic injury via gut microbiota dysbiosis-Induced intestinal barrier disruption.

Journal of environmental management·2026
Same author

Signal Detection of Drug-Drug Interactions Between Topical Vitamin D3 Analogs and Oral Thiazide Diuretics in Hypercalcemia: Analysis of the Japanese Drug Event Report Database.

The Journal of dermatology·2025
Same author

Antibiotics in northern China's largest shallow wetland lake: Occurrence, bioaccumulation, ecological risk, and indicator screening.

Journal of environmental sciences (China)·2025
Same author

Evaluation of an educational program for community pharmacists involved in pharmacotherapy for patients with impaired renal function: a preliminary study.

Clinical and experimental nephrology·2025
Same author

Estrogenic actions of alkaloids: Structural characteristics and molecular mechanisms.

Biochemical pharmacology·2024

Related Experiment Video

Updated: Jul 7, 2026

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
06:07

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

Published on: June 7, 2020

Evaluation of estrogenic activity of phthalate esters by gene expression profiling using a focused microarray

Meher Parveen1, Akio Inoue, Ryota Ise

  • 1Signaling Molecules Research Laboratory, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.

Environmental Toxicology and Chemistry
|February 12, 2008
PubMed
Summary

Phthalate esters, structurally similar to natural estrogens, can disrupt endocrine systems. This study used microarray analysis to show that butylbenzyl phthalate (BBP) most closely mimics estrogenic activity, while other phthalates exhibit varied effects based on their chemical structure.

More Related Videos

Collection of Alfalfa Root Exudates to Study the Impact of Di(2-ethylhexyl) Phthalate on Metabolite Production
06:46

Collection of Alfalfa Root Exudates to Study the Impact of Di(2-ethylhexyl) Phthalate on Metabolite Production

Published on: June 2, 2023

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory
14:13

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory

Published on: January 1, 2018

Related Experiment Videos

Last Updated: Jul 7, 2026

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
06:07

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

Published on: June 7, 2020

Collection of Alfalfa Root Exudates to Study the Impact of Di(2-ethylhexyl) Phthalate on Metabolite Production
06:46

Collection of Alfalfa Root Exudates to Study the Impact of Di(2-ethylhexyl) Phthalate on Metabolite Production

Published on: June 2, 2023

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory
14:13

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory

Published on: January 1, 2018

Area of Science:

  • Environmental Science
  • Toxicology
  • Molecular Biology

Background:

  • Phthalates are industrial plasticizers that contaminate the environment.
  • Their structural similarity to natural estrogens suggests potential endocrine-disrupting activity.
  • Understanding phthalate estrogenicity is crucial for assessing environmental and health risks.

Purpose of the Study:

  • To investigate the estrogenic activity of common phthalate esters.
  • To compare the gene expression profiles of phthalate-treated cells with those of 17beta-estradiol (E[2]).
  • To correlate chemical structure with estrogenic potency using microarray analysis.

Main Methods:

  • Utilized a DNA microarray (EstrArray) to analyze gene expression in MCF-7 cells.
  • Treated cells with butylbenzyl phthalate (BBP), dibutyl phthalate (DBP), diethyl phthalate (DEP), diisopropyl phthalate (DIP), and 17beta-estradiol (E[2]).
  • Performed correlation and cluster analysis on gene expression profiles to quantify estrogenic similarity.

Main Results:

  • Butylbenzyl phthalate (BBP) exhibited the highest correlation (r=0.85) with E[2], indicating strong estrogenic activity.
  • Diethyl phthalate (DEP) and diisopropyl phthalate (DIP) showed moderate correlations (r=0.52, r=0.49), while dibutyl phthalate (DBP) had the lowest significant correlation (r=0.36).
  • Genes in enzyme, transcription, and other functional groups commonly responded to phthalates and E[2], with BBP's effect most closely mirroring E[2].

Conclusions:

  • Chemical structure strongly influences estrogenic activity among phthalate esters.
  • Focused microarray analysis effectively evaluates estrogenic potency by grouping responsive genes.
  • Results highlight differential endocrine-disrupting potential of various phthalates, with implications for risk assessment.