Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

SNP-finding in pig mitochondrial ESTs.

K Scheibye-Alsing1, S Cirera, M J Gilchrist

  • 1Division of Genetics and Bioinformatics, IBHV, University of Copenhagen, Grønnegårdsvej 3, DK-1870 Frederiksberg, Denmark.

Animal Genetics
|February 12, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring the opportunity for therapeutic drug monitoring (TDM) and precision dose antimicrobials in an outpatient antimicrobial therapy (OPAT) service: a prospective observational study.

The Journal of antimicrobial chemotherapy·2026
Same author

The first genome-wide association study concerning idiopathic epilepsy in Petit Basset Griffon Vendeen.

Animal genetics·2021
Same author

Diet-Dependent Changes of the DNA Methylome Using a Göttingen Minipig Model for Obesity.

Frontiers in genetics·2021
Same author

Unraveling molecular mechanisms involved in the development of leptin resistance using the pig as a model.

Animal genetics·2020
Same author

Validation of DNA test for hip dysplasia failed in Danish Labrador Retrievers.

Animal genetics·2020
Same author

Analysis of porcine body size variation using re-sequencing data of miniature and large pigs.

BMC genomics·2018
Same journal

Genome-Wide Association Study and Genomic Prediction for Maximum Litter Size in Sows.

Animal genetics·2026
Same journal

Transcriptomic Analysis Reveals Multilayer Regulation Underlying Development and Pregnancy in the Malayan Pangolin.

Animal genetics·2026
Same journal

Gestational Feed Restriction Induces Differential Expression of Novel Long Non-Coding RNAs in Offspring Skeletal Muscle.

Animal genetics·2026
Same journal

A NECAP1 Nonsense Variant is Associated With Leukoencephalomyelopathy With Oligodendroglial Dysplasia in a Belgian Malinois With Spinocerebellar Ataxia.

Animal genetics·2026
Same journal

Enrichment Analysis of Differentially Expressed Genes During Feather Regeneration in Landes Geese (Anser anser).

Animal genetics·2026
Same journal

Multi-Omics Analysis Reveals Impacts of SCARB1, TYR, and TYRP1 Knockouts on Pigmentation and Metabolic Pathways in Oujiang Color Common Carp.

Animal genetics·2026
See all related articles

Researchers identified 112 high-confidence pig mitochondrial single nucleotide polymorphisms (SNPs) from expressed sequence tags (ESTs). Eight of nine tested SNPs were confirmed, offering valuable genetic markers for pig research.

Area of Science:

  • Genomics
  • Animal Genetics
  • Bioinformatics

Background:

  • The Sino-Danish pig genome project generated a large dataset of expressed sequence tags (ESTs).
  • A significant portion of these ESTs originated from the pig mitochondrial genome, presenting an opportunity for genetic analysis.

Purpose of the Study:

  • To assemble mitochondrial ESTs from the pig genome project.
  • To identify and validate single nucleotide polymorphisms (SNPs) within the pig mitochondrial genome.

Main Methods:

  • Assembly of mitochondrial expressed sequence tags (ESTs).
  • Bioinformatic analysis to detect putative single nucleotide polymorphisms (SNPs).
  • Manual inspection of chromatograms for SNP validation.
  • Experimental confirmation of high-confidence SNPs.

Related Experiment Videos

Main Results:

  • Assembly of mitochondrial ESTs yielded 374 putative SNPs.
  • Manual inspection and validation identified 112 high-confidence SNPs, including 52 non-synonymous variants.
  • Eight out of nine experimentally tested SNPs were confirmed, demonstrating high reliability.
  • Some identified SNPs showed proximity to human disease-associated SNPs.

Conclusions:

  • A robust set of 112 high-confidence mitochondrial SNPs has been identified in pigs.
  • These validated SNPs represent valuable genetic markers for pig genomics and breeding.
  • The identified SNPs are accessible online for broader research use.