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Updated: Jul 7, 2026
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
Published on: September 10, 2014
Electrophoresis PDMS/glass chips with continuous on-chip derivatization and analysis of amino acids using
O Yassine1, P Morin, O Dispagne
1Institut des Nanotechnologies de Lyon, INL, CNRS UMR5270, Université de Lyon, F-69003 Lyon, France.
Abstract:
In this work, we developed a PDMS electrophoresis device able to carry out on-chip derivatization and quantification of amino acids (AAs) using naphthalene-2,3-dicarboxaldehyde (NDA) as a fluorogenic agent. A chemical modification of the PDMS surface was found compulsory to achieve the derivatization of AAs with NDA and a limit of detection (LOD) of 40nM was reached for glycine. Finally, we suggested the applicability of this microdevice for the analysis of real biological samples such as a rat hippocampus microdialysate.
