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Updated: Jul 7, 2026

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
Published on: November 20, 2014
Efficient purification of small unsaturated oligoglucuronides by reversed-phase chromatography
Marie-Laure Tavernier1, Emmanuel Petit, David Lesur
1Laboratoire des Glucides, UMR CNRS 6219, Université de Picardie Jules Verne, 33 rue Saint-Leu, F-80039 Amiens, France.
Abstract:
Ion-exchange chromatography has been applied to purification of unsaturated oligoglucuronans. After an isocratic elution on a strong anion-exchange column, the collected fractions were desalted by low pressure size exclusion chromatography. However, this efficient separation was limited by the time required to desalt. So, we developed a reversed-phase chromatography method using back ionization of oligomers. Two C18 columns were tested with trifluoroacetic acid (TFA 0.7%) as eluent. Different selectivities and column stabilities were observed in this acidic condition. The scale up for semi-preparative applications enabled us to recover pure unsaturated oligoglucuronans without desalting step.

