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Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release
Published on: June 12, 2021
Principles and applications of ultrasound backscatter microscopy
F S Foster1, G R Lockwood, L K Ryan
1Dept. of Med. Biophys., Toronto Univ., Ont.
Summary
Ultrasound backscatter microscopy (UBM) offers high-resolution tissue imaging up to 4 mm deep. This advanced technique visualizes cellular structures and tracks drug uptake in tumors, aiding biological and clinical research.
Area of Science:
- Biomedical Engineering
- Microscopy
- Ultrasound Imaging
Background:
- Ultrasound backscatter microscopy (UBM) builds upon established B-mode imaging techniques.
- Conventional clinical ultrasound operates within the 3-10 MHz frequency range.
- Previous limitations in resolution and penetration hindered detailed tissue analysis.
Purpose of the Study:
- To describe the development of ultrasound backscatter microscopy (UBM).
- To present initial clinical and biological applications of UBM.
- To highlight the advancements in high-frequency transducer technology for enhanced imaging.
Main Methods:
- Development of high-sensitivity transducers operating at 40-100 MHz.
- Implementation of high-frequency ultrasound imaging systems.
- Review of performance characteristics and trade-offs of polymer and ceramic transducers.
Main Results:
- Achieved visualization of tissue structures with resolution approaching 20 µm.
- Demonstrated maximum penetration depth of approximately 4 mm.
- Successfully applied UBM in ophthalmic, skin, and intravascular imaging.
- Illustrated drug uptake in living tumor spheroids, showing increased backscatter in oxic and hypoxic cells.
Conclusions:
- UBM provides unprecedented resolution for microscopic tissue visualization.
- The technology shows promise for diverse clinical applications, including ophthalmology and dermatology.
- UBM is a valuable tool for studying cellular responses to drug treatments in biological models.
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