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Related Concept Videos

Structural Protein Function01:56

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to form...
Structural Protein Function01:56

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to form...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...

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Related Experiment Video

Updated: Jul 7, 2026

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
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Structure/function analysis of yeast ribosomal protein L2.

Arturas Meskauskas1, Johnathan R Russ, Jonathan D Dinman

  • 1Department of Cell Biology and Molecular Genetics, Microbiology Building Rm. 2135, University of Maryland, College Park, MD, 20742, USA.

Nucleic Acids Research
|February 12, 2008
PubMed
Summary

Mutations in ribosomal protein L2 (RPL2A) disrupt yeast ribosome function. These findings reveal L2

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Ribosomal protein L2 (RPL2A) is a conserved, essential component of the large ribosomal subunit.
  • RPL2A interacts extensively with ribosomal RNA (rRNA) and is crucial for ribosome structure and function, including subunit association and catalysis.

Purpose of the Study:

  • To investigate the functional impact of specific mutations in yeast RPL2A on ribosome activity.
  • To elucidate the role of different domains of RPL2A in key ribosomal processes.

Main Methods:

  • Generation and screening of a library of randomly mutated yeast RPL2A alleles.
  • Characterization of translationally defective mutants, including assessment of peptidyltransferase activity, subunit joining, and tRNA binding.
  • Analysis of rRNA structure alterations in proximity to the peptidyltransferase center.

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Main Results:

  • Three translationally defective RPL2A mutants were identified and classified into two groups based on their phenotypes.
  • Mutants V48D and L125Q (globular domain) impaired A-site functions, peptidyltransferase activity, and subunit joining.
  • Mutant H215Y (extended domain) specifically affected peptidyl-tRNA binding and peptidyltransferase activity.
  • Both mutant classes induced rRNA structural changes distant from the mutation site, impacting the A-site of the peptidyltransferase center.

Conclusions:

  • Defective interactions with Helix 55 and Helix 65-66 suggest flexibility in the RPL2A neck region.
  • This flexibility may be essential for coordinating tRNA interactions within the ribosome.
  • RPL2A plays a critical role in maintaining ribosome integrity and catalytic function through domain-specific interactions.