Related Experiment Video
Updated: Jul 7, 2026

10:00
Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
Published on: November 8, 2016
Solid-phase capture of pathogenic bacteria by using gangliosides and detection with real-time PCR
Prerak T Desai1, Marie K Walsh, Bart C Weimer
1Utah State University, Center for Integrated BioSystems, 4700 Old Main Hill, Logan, UT 84322-4700, USA.
Applied and Environmental Microbiology
|February 12, 2008
Abstract:
We developed a method for concentrating pathogens from samples without enrichment. Immobilized gangliosides concentrated bacteria for detection with real-time PCR. A sensitivity of approximately 4 CFU/ml (3 h) in samples without competing microflora was achieved. Samples with competing microflora had a sensitivity of 40,000 CFU/ml. The variance was less than one cycle.

