Generating somatic mosaicism with a Cre recombinase-microsatellite sequence transgene
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Updated: Jul 7, 2026

Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
Published on: November 17, 2017
Aytekin Akyol1, Takao Hinoi, Ying Feng
1Department of Internal Medicine, University of Michigan Medical School, 109 Zina Pitcher, Ann Arbor, Michigan 48109, USA.
Abstract:
Strategies for altering constitutional or somatic genotype in mice are well established, but approaches to generate mosaic genotypes in mouse tissues are limited. We showed that a functionally inactive Cre recombinase transgene with a long mononucleotide tract altering the reading frame was stochastically activated in the mouse intestinal tract. We demonstrated the utility of this approach by inducing colonic polyposis after Cre-mediated bi-allelic inactivation of the Apc gene.
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