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Updated: Jul 7, 2026

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
Published on: November 9, 2017
Study of DNA internal dynamics by quasi-elastic light scattering
Abstract:
We have studied the large-scale internal fluctuations in DNA coils by using quasi-elastic light-scattering spectroscopy. We have measured the angular dependence of the first cumulant of the scattered-light autocorrelation function. Within the q (3) domain of this dependence, we observed the transition from the asymptotic behavior predicted for good solvents to the asymptotic behavior predicted for ? conditions. This allowed us to determine the screening length of volume interactions in DNA chains. Analysis of the autocorrelation functions by a regularization procedure allowed us to reconstruct the mode composition of the scattered light and to determine the relaxation time of the fluctuations in the coil size.
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