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Updated: Jul 7, 2026

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
Published on: May 13, 2020
Synthesis and testing of chromogenic phenoxazinone substrates for beta-alanyl aminopeptidase
Andrey V Zaytsev1, Rosaleen J Anderson, Alexandre Bedernjak
1Pharmacy, Chemistry and Biomedical Sciences, University of Sunderland, Sunderland, SR1 3SD, U.K.
Abstract:
Novel 7-N-(beta-alanyl)aminophenoxazin-3-one salts 27a-d have been synthesized and tested as chromogenic substrates for beta-alanyl aminopeptidase, which is present in Pseudomonas aeruginosa, the most common respiratory pathogen in patients with cystic fibrosis. The biological results show that 7-N-(beta-alanyl)amino-1-pentylphenoxazin-3-one trifluoroacetate salt 27a is a chromogenic substrate for this bacterium, with a low degree of diffusion in nutrient media for growing bacterial cultures and a bright red colour, making it easily distinguishable from the agar background.
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