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Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
11:14

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Published on: November 7, 2018

Complete genomic sequence and phylogenetic relatedness of hepatitis B virus isolates in Cambodia

Tran Thien Tuan Huy1, Amadou Alpha Sall, Jean Marc Reynes

  • 1Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan.

Virus Genes
|February 12, 2008
PubMed

Insights

This study characterizes hepatitis B virus (HBV) strains in Cambodia, finding prevalent HBV/C1 and HBV/B4 genotypes. Molecular analysis reveals genetic recombination in some HBV/B4 isolates, offering new insights into HBV diversity in the region.

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Cambodia is recognized as a high endemic area for hepatitis B virus (HBV) infection.
  • Limited molecular data exists on the HBV strains circulating within Cambodia.

Purpose of the Study:

  • To perform the first molecular characterization of hepatitis B virus (HBV) strains prevalent in Cambodia.
  • To identify the genotypes and subgenotypes of HBV circulating in the Cambodian population.

Main Methods:

  • Sequencing of the pre-S gene from 12 Cambodian HBV isolates.
  • Phylogenetic analysis to determine HBV genotypes.
  • Determination of complete genomic sequences for selected isolates.
  • Recombination analysis using SimPlot and BootScanning.

Main Results:

  • Phylogenetic analysis identified HBV/C1 (66.7%) and HBV/B4 (33.3%) as the predominant HBV subgenotypes.
  • Complete genome sequencing confirmed the presence of both subgenotypes.
  • Two of the sequenced HBV/B4 isolates exhibited recombination with genotype C in the precore/core gene.

Conclusions:

  • Hepatitis B virus (HBV) strains belonging to subgenotypes B4 and C1 are circulating in Cambodia.
  • This study provides the first molecular characterization of HBV in Cambodia.
  • Evidence of genetic recombination between HBV genotypes highlights the complexity of HBV epidemiology in the region.

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