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Updated: Jul 7, 2026
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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
Published on: June 11, 2015
Introduction to vectors derived from filamentous phages
1Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Current Protocols in Molecular Biology
|February 12, 2008
Summary
Filamentous phage vectors, derived from filamentous phages, allow recovery of inserted DNA as double-stranded or single-stranded circles. This overview details their lifecycle, development, and applications in molecular biology.
Area of Science:
- Molecular Biology
- Virology
- Biotechnology
Background:
- Filamentous phages are widely utilized as vectors in molecular biology.
- These phage vectors facilitate the recovery of inserted DNA in both double-stranded and single-stranded circular forms.
Purpose of the Study:
- To provide a comprehensive overview of the filamentous phage lifecycle.
- To describe the development and application of filamentous phage vectors.
Main Methods:
- Review of existing literature on filamentous phage biology.
- Analysis of the mechanisms underlying DNA recovery in double-stranded and single-stranded forms.
Main Results:
- Filamentous phages possess a unique lifecycle amenable to vector development.
- The dual recovery forms (dsDNA and ssDNA circles) offer versatility in molecular cloning and manipulation.
Conclusions:
- Filamentous phage vectors represent a valuable tool in genetic engineering.
- Understanding their lifecycle is crucial for optimizing their use in various biotechnological applications.
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