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Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
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Preparing and using M13-derived vectors
1Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Current Protocols in Molecular Biology
|February 12, 2008
Abstract:
Many vectors in current use are derived from filamentous phages. These vectors are used because DNA inserted into them can be recovered in two forms: double-stranded circles and single-stranded circles. This overview unit describes the lifecycle of filamentous phages along with the development and use of filamentous phage vectors.
