Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel Alzheimer Disease Risk Loci and Pathways in African American Individuals Using the African Genome Resources Panel: A Meta-analysis.

JAMA neurology·2020
Same author

Open-label randomized trial of torsemide compared with furosemide therapy for patients with heart failure.

The American journal of medicine·2001
Same author

A comparison of aorta and vena cava medial message expression by cDNA array analysis identifies a set of 68 consistently differentially expressed genes, all in aortic media.

Circulation research·2000
Same author

Plasminogen activator inhibitor type-1 and interleukin-6 in haemolytic uraemic syndrome.

Journal of paediatrics and child health·2000
Same author

Fas/FADD-mediated activation of a specific program of inflammatory gene expression in vascular smooth muscle cells.

Nature medicine·2000
Same author

CART peptides.

Regulatory peptides·2000

Related Experiment Video

Updated: Jul 7, 2026

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis
08:12

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

Published on: September 28, 2018

Two-dimensional gel electrophoresis using the ISO-DALT system.

L D Adams1

  • 1The Upjohn Company, Kalamazoo, Michigan, USA.

Current Protocols in Molecular Biology
|February 12, 2008
PubMed
Summary

This study combines isoelectric focusing and SDS-polyacrylamide gel electrophoresis for enhanced protein separation. The ISO-DALT system enables high-resolution, simultaneous two-dimensional gel electrophoresis of multiple samples.

Area of Science:

  • Proteomics
  • Biochemistry
  • Analytical Chemistry

Background:

  • Traditional electrophoretic methods offer limited resolution for complex protein mixtures.
  • Separating proteins based on both charge and size is crucial for comprehensive proteomic analysis.

Purpose of the Study:

  • To develop a high-resolution technique for protein separation.
  • To improve upon existing two-dimensional gel electrophoresis methods.
  • To introduce a system capable of processing multiple gels concurrently.

Main Methods:

  • Integration of isoelectric focusing (IEF) and SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
  • Proteins are initially separated by isoelectric point using IEF in a tube gel.
  • The IEF gel is subsequently run on an SDS-PAGE slab gel for separation by molecular size.

More Related Videos

Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers
10:10

Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers

Published on: April 26, 2018

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
12:34

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Published on: April 2, 2018

Related Experiment Videos

Last Updated: Jul 7, 2026

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis
08:12

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

Published on: September 28, 2018

Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers
10:10

Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers

Published on: April 26, 2018

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
12:34

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Published on: April 2, 2018

Main Results:

  • Achieved significantly higher resolution compared to individual electrophoretic techniques.
  • Demonstrated effective separation of solubilized proteins based on both isoelectric point and molecular weight.
  • The ISO-DALT system facilitates simultaneous analysis of multiple two-dimensional gels.

Conclusions:

  • The combined IEF and SDS-PAGE approach provides superior protein separation.
  • The ISO-DALT system is an efficient platform for high-throughput, high-resolution two-dimensional gel electrophoresis.
  • This method enhances the ability to analyze complex proteomes.