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Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Vaccine Production01:23

Vaccine Production

Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...

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Related Experiment Video

Updated: Jul 7, 2026

Laboratory Scale Production and Purification of a Therapeutic Antibody
09:54

Laboratory Scale Production and Purification of a Therapeutic Antibody

Published on: January 24, 2017

In vitro antibody production.

J J Mond1, M Brunswick

  • 1Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.

Current Protocols in Molecular Biology
|February 12, 2008
PubMed
Summary

This study details methods for stimulating B cells to produce antibodies in vitro and quantifying these antibody-producing cells using plaque-forming cell (PFC) assays. These techniques allow for the measurement of all antibody classes and total immunoglobulin-secreting B cells.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • B cells are crucial for adaptive immunity, producing antibodies to neutralize pathogens.
  • Accurate measurement of antibody production is essential for understanding immune responses and developing vaccines.

Purpose of the Study:

  • To describe a generalized system for inducing in vitro antibody production by B cells.
  • To present methods for quantifying antibody-secreting cells using plaque-forming cell (PFC) assays.
  • To detail a protocol for preparing resting B cells.

Main Methods:

  • Antigenic stimulation of B cells in vitro.
  • Quantification of antibody-producing cells via Cunningham-Szenberg and Jerne-Nordin plaque-forming cell (PFC) assays.
  • Percoll gradient centrifugation for B cell preparation.

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Last Updated: Jul 7, 2026

Laboratory Scale Production and Purification of a Therapeutic Antibody
09:54

Laboratory Scale Production and Purification of a Therapeutic Antibody

Published on: January 24, 2017

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
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Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Published on: June 5, 2015

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
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A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

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Main Results:

  • A generalized system for in vitro antibody production was established.
  • Plaque-forming cell (PFC) assays were optimized for quantifying antibody-producing cells.
  • Assays can be modified to measure all antibody classes or total immunoglobulin-secreting B cells.

Conclusions:

  • The described methods provide a robust framework for studying B cell antibody production in vitro.
  • These techniques are valuable for immunological research, vaccine development, and diagnostics.
  • Optimized B cell preparation and PFC assays enhance the accuracy of antibody quantification.