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Updated: Jul 7, 2026
09:42
Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
Cloning of hybridoma cell lines by limiting dilution
S A Fuller1, M Takahashi, J G Hurrell
1Allelix Inc., Mississauga, Ontario.
Current Protocols in Molecular Biology
|February 12, 2008
Abstract:
Cloning by limiting dilution is a method based on the Poisson distribution. Dilution of cells to an appropriate number per well can maximize the proportion of wells that contain one single clone. Two or more cloning procedures are carried out until >90% of the wells containing single clones are positive for antibody production.
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01:31Hybridoma Technology
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01:16Cell Lines
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...