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Direct DNA sequencing of PCR products

R L Dorit1, O Ohara, C B Hwang

  • 1Yale University, New Haven, Connecticut, USA.

Current Protocols in Molecular Biology
|February 12, 2008
PubMed
Summary

This study presents methods for DNA sequencing using asymmetric PCR to generate single-stranded templates for Sanger sequencing. It also details preparing PCR products for genomic and chemical sequencing of end-labeled DNA.

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