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Molecular cloning of PCR products
M Finney1, P E Nisson, A Rashtchian
1MJ Research, Watertown, Massachusetts, USA.
Current Protocols in Molecular Biology
|February 12, 2008
Abstract:
It is often desirable to clone PCR products to establish a permanent source of cloned DNA for hybridization studies, to obtain high-quality DNA sequencing results, or to separate products when PCR amplification yields a complex mixture. The efficiency of direct cloning of PCR products can be improved by generating suitable ends on the amplified fragments. This unit describes the strategies for generating and manipulating suitable ends on the PCR fragments.
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