Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

cDNA amplification using one-sided (anchored) PCR.

R L Dorit1, O Ohara

  • 1Yale University, New Haven, Connecticut, USA.

Current Protocols in Molecular Biology
|February 12, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Glial reactivity and cognitive decline follow chronic heterochromatin loss in neurons.

Nature communications·2025
Same author

Analysis of Mutations in the IL2RG Gene in 2 Asian Infants With X-linked Severe Combined Immunodeficiency.

Journal of investigational allergology & clinical immunology·2015
Same author

Successful treatment of systemic cytomegalovirus infection in severe combined immunodeficiency using allogeneic bone marrow transplantation followed by adoptive immunotherapy.

Journal of investigational allergology & clinical immunology·2014
Same author

Chronic granulomatous disease: two decades of experience from a tertiary care centre in North West India.

Journal of clinical immunology·2013
Same author

Novel mutation of IL2RG gene in a Korean boy with X-linked severe combined immunodeficiency.

Journal of investigational allergology & clinical immunology·2013
Same author

Severe primary antibody deficiency due to a novel mutation of mu heavy chain.

Journal of investigational allergology & clinical immunology·2012
Same journal

Nondenaturing Polyacrylamide Gel Electrophoresis: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Purification and Concentration of DNA from Aqueous Solutions: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Expression of Proteins Using Semliki Forest Virus Vectors: Protein Expression.

Current protocols in molecular biology·2021
Same journal

Methylation and Uracil Interference Assays for Analysis of Protein-DNA Interactions: DNA-Protein Interactions.

Current protocols in molecular biology·2021
Same journal

Separation of Double- and Single-Stranded Nucleic Acids Using Hydroxylapatite Chromatography: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Pulsed-Field Gel Electrophoresis: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
See all related articles

Anchored PCR enables full-length mRNA amplification using limited sequence data. This method overcomes limitations of conventional PCR, which requires flanking sequences for primer design.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Conventional Polymerase Chain Reaction (PCR) requires knowledge of sequences flanking the target region for primer design.
  • Isolating specific mRNA sequences can be challenging with limited initial sequence information.

Purpose of the Study:

  • To describe anchored PCR, a modified technique for amplifying full-length mRNA.
  • To enable the amplification of target mRNA with minimal known sequence data.

Main Methods:

  • Utilizes anchored PCR, a modification of standard PCR.
  • Requires only a small region of known sequence within the mRNA of interest.

Main Results:

  • Anchored PCR allows for the amplification of full-length mRNA.

Related Experiment Videos

  • This technique is effective even with limited initial sequence information.

    • Conclusions:

    • Anchored PCR provides a powerful method for mRNA amplification when sequence information is scarce.
    • It expands the utility of PCR for isolating and studying specific RNA molecules.