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Updated: Jul 7, 2026

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
Published on: November 20, 2014
Beta-elimination for release of O-linked glycosaminoglycans from proteoglycans
1University of Illinois, Urbana, Illinois, USA.
Abstract:
O-linked glycosaminoglycan (GAG) chains in proteoglycans are readily released from their core proteins by treatment with alkali at room temperature. This beta-elimination is the same type of reaction as that for releasing O-linked oligosaccharides from their core proteins. Under the reaction conditions described here, N-linked oligosaccharides remain attached to the core protein, but any O-linked oligosaccharides will be released along with the GAG chains. The procedure can be used to isolate the free GAG chains, the free O-linked oligosaccharides, and the core protein (which will still have any N-linked oligosaccharides that were originally present).
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