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Updated: Jul 7, 2026

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
Published on: November 20, 2014
Beta-elimination for release of O-GalNAc-linked oligosaccharides from glycoproteins and glycopeptides
1La Jolla Cancer Research Foundation, La Jolla, California, USA.
Abstract:
This unit describes release of oligosaccharides that are attached to polypeptides through an N-acetylgalactosamine (GalNAc) linkage to the hydroxyl groups of serine or threonine. The beta-elimination procedures described here can be used to recover the oligosaccharide chains (also called glycans) and/or identify the serine or threonine residues involved in the linkage. A beta-elimination method employing sodium borohydride (NaBH4) and alkaline conditions is described, and an alternative method is also presented in which only alkaline conditions are used without a reducing agent. Another alternative protocol uses sodium sulfite.
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