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Fractionation and analysis of neutral oligosaccharides by HPLC

B K Hayes1, A Varki

  • 1University of Alabama at Birmingham, Birmingham, Alabama, USA.

Current Protocols in Molecular Biology
|February 12, 2008
PubMed

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Oligosaccharide Assembly01:24

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Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...